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KMID : 0353320020260020297
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Oral Biology Research
2002 Volume.26 No. 2 p.297 ~ p.304
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Recovery step may be ommitted in transformation method
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Abstract
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We previously introduced the direct transformation method, direct plating of transformation mixture onto LB agar plate after immediately mixing of competent cells and pUC19 plasmid DNA. In that report, the difference of transformation efficiency according to the recovery time of transformed E. coli with pUC19 was not determined. In this study, to completely establish the direct transformation method, transformation efficiency according to the recovery time of transformed E. coli was determined. Our data reveal that the transformation efficiency was increased the recovery time after heat shock. However, this result come from the mitosis of transformed E. coli with pUC19. Our data indicate recovery step may be omitted in transformation experiment.
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